What is 260 and 280 ratio
Absorbance ratios 260/280 and 260/230 for RNA
DNA and RNA absorb at 260 nm. Proteins absorb at 280 nm. The 260/280 ratio is a good estimate of how pure your sample is. For RNA, the 260/280 should be around 2. If it is lower, it could be an indication of contamination or proteins, phenol or other contaminants in your sample. The 260/230 ratio is a second measure of the purity of the sample, as the impurities absorb at 230 nm (like EDTA). The 260/230 ratio should be higher than the 260/280 ratio as it is usually between 2 and 2.2. A lower ratio can indicate contamination.
In your case I would be concerned about the purity of the sample which gave you a 260/230 ratio of 0.98. If you are using nanodrops, a look at the diagram can also be a good indication of the quality of your sample. For a pure sample, a well-defined peak (no shoulders or wobbling) is expected at 260 nm.
Please refer to the NanoDrop manual for more information.
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